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Ships within 48 hours · Estimated delivery Jul 4 - Jul 9
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Description
Mouse IL-2sRAlpha/CD25 (Soluble Interleukin-2 Receptor Alpha Chain) ELISA KitThe Mouse IL 2sRAlpha CD25 (Soluble Interleukin 2 Receptor Alpha Chain) ELISA Kit measures IL 2sRAlpha CD25 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 2sR CD25 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation,
The Mouse IL-2sRAlpha/CD25 (Soluble Interleukin-2 Receptor Alpha Chain) ELISA Kit measures IL-2sRAlpha/CD25 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-2sRα/CD25 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-2sRα/CD25 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-2sRα/CD25 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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